The analysis of the stool, the worms and eggs, not - end - directions for the research

Currently, the analysis of the faeces, worms is the routine of a diagnostic laboratory handling, assigning children and adults, both in the scope of the research, with the goal of identifying the pathological causes of the symptoms and during the preventive maintenance the annual examination. This analysis, in spite of the wide prevalence, it is not always clear to people, I therefore consider the purpose, the methodology for conducting and interpreting the results.

Research on enterobiasis

The analysis of the stool, worm eggs, worm) – a common feature of

The analysis of the faeces, worms properly called analysis of eggs end-to-end, since during the execution of the manipulation of an official diagnosis by laboratory investigates the stool to verify the presence of eggs of a variety of parasitic worms that can reach the organs and tissues of the human body. And because the collective name of all the parasitic worms – this worms, the main aim of the analysis is to detect or confirm the lack of eggs data of parasites.

The fact is that in various organs and tissues of the human body can parasite about 250 species of worms (worms), and they reproduce, leaving eggs. Exactly these eggs fall off in the intestine, and stand out to the outside along with the stool. The study of the smear fecal matter under the microscope allows you to see with the naked eye the larvae and eggs of various parasitic worms, thus, the diagnosis of its presence. That is, the analysis of the feces, the eggs end-to-end is a method of diagnosis of helminths diseases caused by parasitic worms (worms).

Any type of parasites (worms) can reveal through an examination of the stool?

During currently used in the laboratory techniques of analysis of the feces, the eggs end-to-end, can reveal the presence of the following parasitic worms (worms):

The class of roundworms (ancilostomídeos):

  • The roundworm (Ascaris lumbricoides (roundworm));
  • The genus meloidogyne (Trichocephalus trichiurus);
  • Thominx aerofilus;
  • Ancylostoma duodenale;
  • Necator (Necator americanus);
  • Strongyloides stercoralis larvae.

The class are flukes (trematodes-worms):

  • Plaice liver (Fasciola liver);
  • Plaice feline (Opisthorchis felineus);
  • Lancet fluke lanceatum;
  • Schistosoma mansoni et japonicum.

The class of worms (cestodes):

  • Tapeworm nu (Taeniarhynchus saginatus);
  • Tapeworm armed (Taenia solium);
  • A large tapeworm (Diphyllobothrium diphyllobotrium);
  • Tapeworm (Diphyllobothrium minus).
Diagnosis

That is, all of these species of parasitic worms can be found through the normal process of analysis of the feces, the eggs end-to-end. It should be understood that in the stool are not detected if helminths, at a fraction of their shells, or eggs, delayed for playback. So, if the doctor sees in the feces the eggs of any parasites worms, this means that delay could only helminths, and therefore, he somehow fell inside the body of this individual. That is, if there are eggs of helminthes in the stool can be with 100% certainty that the person is infected data parasite a worm, which it is necessary to bring the body.

In addition to the worms on the analysis of the feces, the eggs end-to-end, it is possible to identify the infection protozoa, such as:

  • Dysentery amoeba (Entamoebahistolytica);
  • Giardia (Giardialamblia).

Also in the stool can be detected with cyclosporine and their larvae.

When you need to do the examination of the feces the eggs a worm (signs of search)

The analysis of the feces, the eggs end-to-end for the two possible cases:

A suspected infestation of worms (helminthiasis);

Barrier analysis.

Barrier analysis is a requirement of the study is included in the routine procedure of the research are the people who come in work in connection with the sale, handling or storage of food products, contact with people and other areas of operation, that can transmit the worms to the other. In this situation, the analysis of the feces, the eggs end-to-end is screening, or is, used for the mass and a quick diagnosis of helminths of many people. In a nutshell one can say that as the required barrier analysis of feces for eggs, a worm surrenders to the decoration of portable sanitation and when he arrives in kindergarten, the school, to the swimming pool or before a planned hospital stay in the hospital. This analysis should deliver, provided that the person is no longer in contact with the people, and if he is infected with the helmintíaseom, you can infect other people, thus extending the disease.

The second indication to place the analysis of the feces, the eggs end-to-end is suspected of being infected by any worms. Suspected helminthiasis (infection parasites worms) occurs in the presence of a person of several variable symptoms of operational failure of various organs, in the absence of the respective disease. The fact is that parasitic worms (helminths) can reach various organs and tissues, for example, the intestine, the bladder, the urethra, brain, liver, heart, lymph nodes, and other, causing symptoms of various diseases. If the search is not detected, the disease of the organ, with the side for which there are pathological symptoms, there is the natural suspicion of helminthiasis, which requires the confirmation or refutation. Precisely with the aim to confirm or refute the helmintíasea gives up the examination of the feces the eggs of a worm to such a situation.

Preparation for the analysis of the stool for worms

The analysis of the feces, the eggs end-to-end it shows the deposit, in the presence of a person with the following symptoms:

  • Inflammatory disease organs of the gastrointestinal tract (for example, pain in the stomach, diarrhea, constipation, flatulence, and t. d.);
  • Fatigue;
  • Irritability;
  • It is disturbing the sleep;
  • Bruxism (gnashing teeth);
  • Itching in the anus;
  • Vulvovaginitis in women;
  • Urinary tract infection;
  • The delay in growth and body mass growth of children and adolescents;
  • The increase in the number of eosinophils in the blood;
  • Allergic to syndrome dark of nature, which it is not possible to identify the allergen, call a pathological reaction on the part of the body;
  • Frequent colds, illnesses and viral infections.

If you have any of the symptoms listed the need for an examination of the feces the eggs end-to-end, because they are factors sufficient for the suspicion of helminthiasis. However, only the analysis of the stool may not be sufficient for the diagnosis of helmintíasea, and, in the case of a suspected presence of parasites would have to make samples of bile, saliva, dUodanalnogo content and other secrets of the biological human body, which may be the eggs or larvae of parasitic worms.

Preparation for the analysis of the stool for worms

Before the distribution of the faeces, the eggs end-to-end should be during the week not to take castor or mineral oil, antibiotics, abouttIvodAranya and antiparasitic funds, as well as drugs of bismuth and magnesium. Also within 7 – 14 days prior to the distribution of the faeces, the eggs end-to-end is not advisable to irrigoscopy.

In addition, it is necessary that, psychologically and mentally adjusted to the fact that, for the quality of the diagnosis of helminths will need one-on-one, three or six samples of stool, you will have to pass every day or in 1 – 2 days. Usually you will need three stool samples, but if there is a suspicion of dysentery amoeba or Giardia, an ideal way to spend six stool samples.

It is advisable that you prepare the dishes for the experimentation of the stool. Currently, already are not used, so the usual many people from their childhood, boxes of matches, such as the lab to give special plastic containers with cover. In the lab container not issued, you can buy in the pharmacy's usual. It is best to use exactly the type of a disposable container, and not any existing in the house, the dishes or the box, as they may be the larvae or eggs of parasites, declared, accidentally, dirty hands, that during the research will be identified and, in this way, the person will receive a false positive result.

The lab

More of no special preparation is to pass the analysis of the feces, the eggs an end-to-end produce do not need.

How to properly assemble the cal for analysis of eggs end-to-end?

Before deficacia need to urinate, so that urine does not come into contact with the fecal mass. Then, you should, not my skin anal, pokakatü in any capacity, this location will win samples of the stool (for example, pan, taz, and t. d.).

From the whole mass of the feces, you should collect a small quantity of about two tea spoons, apart from the experimentation with different parts of the stool wand special. If the container for cala, no, you can collect samples of routine phosphorus or a wand. Ideal to make 3 to 4 small pieces of the sides, the front and rear parts of the top surface, as well as the urban masses fecal. All together part cala is placed in a container firmly and close the lid. To the container to adhere a piece of paper with the surname, name and initials, and quickly transported to the laboratory.

For the most accurate results of the ideal way of passing samples of the feces the eggs of a worm to the laboratory within 30 to 45 minutes after defecation, and your fence. However, if within a short period of to provide a stool sample to the laboratory is impossible, you can do so within a maximum of 5 to 8 hours, necessarily, keeping the stool in the refrigerator at temperature +4 +8theWith. If the stool sample for more than 60 minutes remained in the fridge, and in the heat of the room, so they are not suitable for the deposit of the eggs of the worm. In this case, will have to throw out spoiled experimentation and mount again after defecation.

For a more accurate and comprehensive diagnosis should pass only three stool samples. And collect samples can be daily or 1 – 2 days. The final result of the search can be obtained only after a search of three samples.

The result of the analysis of the feces, the eggs end-to-end and decryption

Normal the result of the analysis of the feces, the eggs end-to-end in all three samples must be negative. This means that the eggs of helminths are not found, and the individual is not infected with parasitic worms.

A positive result in at least one of the stool samples is an indicator that the man has discovered helminths, and, therefore, he is infected with parasitic worms. In such a situation, it is necessary to do the treatment and after a month after delivering the re-analysis of the stool.

If the result of the analysis of the feces, the eggs end-to-end positive, he indicates which is exactly the type of parasitic helminths was discovered. The estimated positive formulation the analysis of the feces, the eggs end-to-end is the following: "Positively, eggs ackapud". In each laboratory, the formulation can vary a little, but it necessarily will be present the term "positive" to indicate the presence of worm parasites, and the name of the species of helminths, the eggs that have been discovered.

Which methods that use the laboratory for conducting analysis of the feces, the eggs end-to-end?

The identification of worm parasites in stool specimens in the laboratory carry out using the methods moprovskaja (helminthologia), that is, the detection of eggs of helminths. In laboratories for the diagnosis of parasites in humans typically use three main methods moprovskaja :

    The container
  1. The microscopy of ontdevelopment stain;
  2. The Method Fulleborn;
  3. The Method Of Telemann.

Typically, the stool sample of a consistent way to expose all the three methods moprovskaja . And mainly produce microscopy ontdevelopment of the stain, and then, in random order, do the experimentation Fulleborn and Telemann.

The microscopy of ontdevelopment stain

The microscopy of ontdevelopment stain is careful ItRivonia under the microscope, small droplets of feces, smeared a thin layer of predIUtnome glass. For this, the glass is first applied a drop of 50% glycerin, which facilitates the uniform distribution of the material. Then, take a small piece of stool and mixed with distilled water until the state of sour cream. From the final weight of the cala charge a small blister, put it on the glass and mixed wood stick with glycerin. Then, in this same wand drop smeart on a glass surface with a thin layer. Then on top of the stain put cover glass and consider the material under the microscope.

Experimentation Fulleborn

Experimentation Fulleborn is the mixture of feces with salt water, which is high-density and specific weight, on account of which the eggs of helminths to appear on its surface. For the realization of samples of 10 g of feces stirring with 100 ml of 50% solution of kitchen salt. The solution serves the following 450 – 500 g of salt dissolved in 1 liter of water, heat it to a temperature of 50 – 70theWith.

The mixture of feces with salt water put it in a quiet place, for 1 to 1.5 hours, the end of eggs of helminths to the surface, to the surface of the solution. After the necessary in the amount of time that appears on the surface of a film to relieve the tie and cause it on a glass slide. The film close to the glass cover face and study under the microscope.

Experimentation Telemann

Analysis

Experimentation Telemann is a mixture of faeces hydrochloric acid and ether, which highlight the eggs of intestinal worms. For the production of experimentation Telemann a small amount of stool mixed with 3 – 4 ml of 30% hydrochloric acid and 15 to 20 ml of distilled water. Then, the suspension of cala add 1 – 1.5 ml of ether, mixed well and close the vessel tight whentEPtOh or rubber cap. Then, the boat several times, as you tilt the neck down, stirring the contents. After that, the suspension filtered through a layer of gauze and pricestRifugiot. Obtained after the centrifugation, the precipitate is placed on a glass slide, close the cover glass face and study under the microscope.

If at least one trial were found eggs of helminths, the test result is considered positive.

The analysis of the stool, not-for – of where to spend?

The examination of feces for worms, you can, in any clinical-diagnostic laboratory in the city on a clinic or hospital. However, for the true result, it is recommended to make the examination of feces specialized bacteriological laboratories, which are available when sanitary and epidemiological service of each city or region.

In addition, the examination may be deprived of the laboratory or clinic that has a license to perform such of the diagnostic investigation.

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